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il 13  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec il 13
    Il 13, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/il+13/Human+IL-13%2C+research+grade/pmc12924898-252-39-40
    Average 94 stars, based on 6 article reviews
    il 13 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Monocyte infiltration induces CNS arginine catabolism to fuel neuroinflammation.
    Article Snippet: Three days later, cells were washed once with prewarmed PBS and detached using prewarmed CellStripper (Corning, 15313661). .. Cells were replated at a concentration of 1 million cells per ml in 12- or 24-well plates (Costar, 3737) and stimulated with 100 ng ml−1 LPS (Invivogen, tlrl-3pelps), 20 ng ml−1 IFNγ (Miltenyi Biotec, 130-105-774), 20 ng ml−1 GM-CSF (R&D Systems, 415-ML-050), 20 ng ml−1 IL-4 (Miltenyi Biotec, 130-097-761) and 20 ng ml−1 IL-13 (Miltenyi Biotec, 130-094-070). ..

    Recombinant:

    Article Title: The Differential Effects of Immunosuppressants on Hepatitis E Virus Replication and the Triggered Inflammatory Responses in Macrophages.
    Article Snippet: Human monocytic THP- 1 cells were cultured in RPMI 1640 medium supplemented with 30 ng/mL of phorbol 12- myristate 13- acetate (PMA, Sigma- Aldrich Chemie BV) at 37°C for 48 h to generate M0 macrophages. .. Following PBS washing, M0 macrophages were treated with 100 ng/ mL LPS (Lipopolysaccharides from Escherichia coli O55:B5, Merck Life Science NV) and 25 ng/mL IFN- γ (Recombinant Human IFN- γ, Immunotools) for M1 macrophages polarisation or 25 ng/mL IL- 4 (Miltenyi biotec) and IL- 13 (Miltenyi biotec) for M2 macrophages polarisation for an additional 2 days. .. The morphology of THP- 1 macrophages was assessed by transmission microscopy (EVOS FL Cell Imaging System, ThermoFisher).

    Article Title: Syndecan-3 positively regulates the pro-inflammatory function of macrophages.
    Article Snippet: .. The following day, differentiated THP-1 macrophages were polarized adding recombinant human IFNγ (100 ng/mL, Biolegend Cat. #: 570206), IL-4 (20 ng/ml, Miltenyi Biotec S.L Cat. #: 130-112-411), or IL-13 (20 ng/ ml, Miltenyi Biotec S.L Cat. #: 130-094-117) for a further 24, 48–72 h. Knockout of SDC3 by CRISPR/Cas9 Deletion of SDC3 in THP-1 cells was performed using the following TrueGuide Synthetic gRNAs (sgRNAs) from ThermoFisher Scientific: CRISPR923443_SGM TrueGuide Synthetic sgRNA SDC3 (target DNA sequence: A A C T G G A T G A C C T C T A C T C G Cat. #: A35511) targeting the exon 2 of human SDC3 gene. .. Ribonucleoprotein (RNP) complexes were generated with 30 pmol of TrueCut Cas9 protein v2 (Invitrogen Cat. #: A36496) and 30 pmol of sgRNA.

    Article Title: The Differential Effects of Immunosuppressants on Hepatitis E Virus Replication and the Triggered Inflammatory Responses in Macrophages
    Article Snippet: Human monocytic THP‐1 cells were cultured in RPMI 1640 medium supplemented with 30 ng/mL of phorbol 12‐myristate 13‐acetate (PMA, Sigma‐Aldrich Chemie BV) at 37°C for 48 h to generate M0 macrophages. .. Following PBS washing, M0 macrophages were treated with 100 ng/mL LPS (Lipopolysaccharides from Escherichia coli O55:B5, Merck Life Science NV) and 25 ng/mL IFN‐γ (Recombinant Human IFN‐γ, Immunotools) for M1 macrophages polarisation or 25 ng/mL IL‐4 (Miltenyi biotec) and IL‐13 (Miltenyi biotec) for M2 macrophages polarisation for an additional 2 days. .. The morphology of THP‐1 macrophages was assessed by transmission microscopy (EVOS FL Cell Imaging System, ThermoFisher).

    other:

    Article Title: Mito-Genipin, a Novel Mitochondria-Targeted Genipin Derivative Modulates Oxidative Stress and Inflammation in Macrophages
    Article Snippet: Alternatively, activated macrophages were polarized with 20 ng/mL of IL-4 (130-097-761, Miltenyi Biotec) and 20 ng/mL IL-13 (130-094-070, Miltenyi Biotec) in the presence or absence of 10 μM, 20 μM, and 40 μM, mito-genipin or genipin.

    Knock-Out:

    Article Title: Syndecan-3 positively regulates the pro-inflammatory function of macrophages.
    Article Snippet: .. The following day, differentiated THP-1 macrophages were polarized adding recombinant human IFNγ (100 ng/mL, Biolegend Cat. #: 570206), IL-4 (20 ng/ml, Miltenyi Biotec S.L Cat. #: 130-112-411), or IL-13 (20 ng/ ml, Miltenyi Biotec S.L Cat. #: 130-094-117) for a further 24, 48–72 h. Knockout of SDC3 by CRISPR/Cas9 Deletion of SDC3 in THP-1 cells was performed using the following TrueGuide Synthetic gRNAs (sgRNAs) from ThermoFisher Scientific: CRISPR923443_SGM TrueGuide Synthetic sgRNA SDC3 (target DNA sequence: A A C T G G A T G A C C T C T A C T C G Cat. #: A35511) targeting the exon 2 of human SDC3 gene. .. Ribonucleoprotein (RNP) complexes were generated with 30 pmol of TrueCut Cas9 protein v2 (Invitrogen Cat. #: A36496) and 30 pmol of sgRNA.

    CRISPR:

    Article Title: Syndecan-3 positively regulates the pro-inflammatory function of macrophages.
    Article Snippet: .. The following day, differentiated THP-1 macrophages were polarized adding recombinant human IFNγ (100 ng/mL, Biolegend Cat. #: 570206), IL-4 (20 ng/ml, Miltenyi Biotec S.L Cat. #: 130-112-411), or IL-13 (20 ng/ ml, Miltenyi Biotec S.L Cat. #: 130-094-117) for a further 24, 48–72 h. Knockout of SDC3 by CRISPR/Cas9 Deletion of SDC3 in THP-1 cells was performed using the following TrueGuide Synthetic gRNAs (sgRNAs) from ThermoFisher Scientific: CRISPR923443_SGM TrueGuide Synthetic sgRNA SDC3 (target DNA sequence: A A C T G G A T G A C C T C T A C T C G Cat. #: A35511) targeting the exon 2 of human SDC3 gene. .. Ribonucleoprotein (RNP) complexes were generated with 30 pmol of TrueCut Cas9 protein v2 (Invitrogen Cat. #: A36496) and 30 pmol of sgRNA.

    Sequencing:

    Article Title: Syndecan-3 positively regulates the pro-inflammatory function of macrophages.
    Article Snippet: .. The following day, differentiated THP-1 macrophages were polarized adding recombinant human IFNγ (100 ng/mL, Biolegend Cat. #: 570206), IL-4 (20 ng/ml, Miltenyi Biotec S.L Cat. #: 130-112-411), or IL-13 (20 ng/ ml, Miltenyi Biotec S.L Cat. #: 130-094-117) for a further 24, 48–72 h. Knockout of SDC3 by CRISPR/Cas9 Deletion of SDC3 in THP-1 cells was performed using the following TrueGuide Synthetic gRNAs (sgRNAs) from ThermoFisher Scientific: CRISPR923443_SGM TrueGuide Synthetic sgRNA SDC3 (target DNA sequence: A A C T G G A T G A C C T C T A C T C G Cat. #: A35511) targeting the exon 2 of human SDC3 gene. .. Ribonucleoprotein (RNP) complexes were generated with 30 pmol of TrueCut Cas9 protein v2 (Invitrogen Cat. #: A36496) and 30 pmol of sgRNA.



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    Effects of CuONPs on allergic inflammation and oxidative stress in OVA-induced asthmatic mice. (A) Airway hyperresponsiveness assessed as total respiratory system resistance in response to methacholine challenge (10, 20, and 40 mg/mL). (B–F) Total and differential inflammatory cell counts in BALF. (G–L) IL-1β, IL-6, TNF-α, IL-4, IL-5, <t>and</t> <t>IL-13</t> levels in BALF, measured by ELISA. (M and N) Total IgE and OVA specific IgE levels in serum. (O and P) MDA levels and SOD activity in lung tissue. Data are presented as means ± SD (n = 6 mice/group). ∗∗ p < 0.01 and ∗∗∗ p < 0.001 versus NC group. # p < 0.05, ## p < 0.01, and ### p < 0.001 versus OVA group.
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    Image Search Results


    Effects of CuONPs on allergic inflammation and oxidative stress in OVA-induced asthmatic mice. (A) Airway hyperresponsiveness assessed as total respiratory system resistance in response to methacholine challenge (10, 20, and 40 mg/mL). (B–F) Total and differential inflammatory cell counts in BALF. (G–L) IL-1β, IL-6, TNF-α, IL-4, IL-5, and IL-13 levels in BALF, measured by ELISA. (M and N) Total IgE and OVA specific IgE levels in serum. (O and P) MDA levels and SOD activity in lung tissue. Data are presented as means ± SD (n = 6 mice/group). ∗∗ p < 0.01 and ∗∗∗ p < 0.001 versus NC group. # p < 0.05, ## p < 0.01, and ### p < 0.001 versus OVA group.

    Journal: Redox Biology

    Article Title: Nrf2 pathway mediates copper oxide nanoparticle-induced exacerbation of allergic asthma

    doi: 10.1016/j.redox.2026.104180

    Figure Lengend Snippet: Effects of CuONPs on allergic inflammation and oxidative stress in OVA-induced asthmatic mice. (A) Airway hyperresponsiveness assessed as total respiratory system resistance in response to methacholine challenge (10, 20, and 40 mg/mL). (B–F) Total and differential inflammatory cell counts in BALF. (G–L) IL-1β, IL-6, TNF-α, IL-4, IL-5, and IL-13 levels in BALF, measured by ELISA. (M and N) Total IgE and OVA specific IgE levels in serum. (O and P) MDA levels and SOD activity in lung tissue. Data are presented as means ± SD (n = 6 mice/group). ∗∗ p < 0.01 and ∗∗∗ p < 0.001 versus NC group. # p < 0.05, ## p < 0.01, and ### p < 0.001 versus OVA group.

    Article Snippet: BALF was centrifuged at 300× g for 10 min at 4 °C, and the supernatant was stored for cytokine analysis using commercially available enzyme-linked immunosorbent assay (ELISA) kits to quantify IL-1β, IL-6, tumor necrosis factor (TNF)-α, IL-4, IL-5, and IL-13 (R&D Systems, Minneapolis, MN, USA; Cat. No. MLB00C, M6000B, MTA00B, M4000B, M5000, and M1300CB, respectively).

    Techniques: Enzyme-linked Immunosorbent Assay, Activity Assay

    Effects of Nrf2 overexpression on allergic inflammation and oxidative stress in CuONP-exposed asthmatic mice. (A) Immunofluorescence analysis of lung tissue from mice administered PBS or AAV2/8-GFP via intratracheal instillation. (B) Airway hyperresponsiveness assessed as total respiratory system resistance in response to methacholine challenge (10, 20, and 40 mg/mL). (C–G) Total and differential inflammatory cell counts in BALF. (H–M) IL-1β, IL-6, TNF-α, IL-4, IL-5, and IL-13 levels in BALF, measured by ELISA. (N and O) Total IgE and OVA specific IgE levels in serum. (P and Q) MDA levels and SOD activity in lung tissue. Data are presented as means ± SD (n = 3 mice/group for panels A; n = 6 mice/group for panels B–Q). In panel B, ## p < 0.01 indicates significant differences between GFP-NC and GFP-OVA, and ∗∗ p < 0.01 indicates significant differences between GFP-OVA + CuONPs and Nrf2-OVA + CuONPs. For panels C–Q, ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001 indicate significant differences between AAV-GFP and AAV-Nrf2 within each condition.

    Journal: Redox Biology

    Article Title: Nrf2 pathway mediates copper oxide nanoparticle-induced exacerbation of allergic asthma

    doi: 10.1016/j.redox.2026.104180

    Figure Lengend Snippet: Effects of Nrf2 overexpression on allergic inflammation and oxidative stress in CuONP-exposed asthmatic mice. (A) Immunofluorescence analysis of lung tissue from mice administered PBS or AAV2/8-GFP via intratracheal instillation. (B) Airway hyperresponsiveness assessed as total respiratory system resistance in response to methacholine challenge (10, 20, and 40 mg/mL). (C–G) Total and differential inflammatory cell counts in BALF. (H–M) IL-1β, IL-6, TNF-α, IL-4, IL-5, and IL-13 levels in BALF, measured by ELISA. (N and O) Total IgE and OVA specific IgE levels in serum. (P and Q) MDA levels and SOD activity in lung tissue. Data are presented as means ± SD (n = 3 mice/group for panels A; n = 6 mice/group for panels B–Q). In panel B, ## p < 0.01 indicates significant differences between GFP-NC and GFP-OVA, and ∗∗ p < 0.01 indicates significant differences between GFP-OVA + CuONPs and Nrf2-OVA + CuONPs. For panels C–Q, ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001 indicate significant differences between AAV-GFP and AAV-Nrf2 within each condition.

    Article Snippet: BALF was centrifuged at 300× g for 10 min at 4 °C, and the supernatant was stored for cytokine analysis using commercially available enzyme-linked immunosorbent assay (ELISA) kits to quantify IL-1β, IL-6, tumor necrosis factor (TNF)-α, IL-4, IL-5, and IL-13 (R&D Systems, Minneapolis, MN, USA; Cat. No. MLB00C, M6000B, MTA00B, M4000B, M5000, and M1300CB, respectively).

    Techniques: Over Expression, Immunofluorescence, Enzyme-linked Immunosorbent Assay, Activity Assay